|Title||Mutations in DSTYK and dominant urinary tract malformations.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Sanna-Cherchi, S, Sampogna, RV, Papeta, N, Burgess, KE, Nees, SN, Perry, BJ, Choi, M, Bodria, M, Liu, Y, Weng, PL, Lozanovski, VJ, Verbitsky, M, Lugani, F, Sterken, R, Paragas, N, Caridi, G, Carrea, A, Dagnino, M, Materna-Kiryluk, A, Santamaria, G, Murtas, C, Ristoska-Bojkovska, N, Izzi, C, Kacak, N, Bianco, B, Giberti, S, Gigante, M, Piaggio, G, Gesualdo, L, Vukic, DKosuljandi, Vukojevic, K, Saraga-Babic, M, Saraga, M, Gucev, Z, Allegri, L, Latos-Bielenska, A, Casu, D, State, M, Scolari, F, Ravazzolo, R, Kiryluk, K, Al-Awqati, Q, D'Agati, VD, Drummond, IA, Tasic, V, Lifton, RP, Ghiggeri, GMarco, Gharavi, AG|
|Journal||N Engl J Med|
|Date Published||2013 Aug 15|
|Keywords||Adult, Animals, Base Sequence, Child, Exome, Female, Gene Knockdown Techniques, Genetic Linkage, Genome-Wide Association Study, Heterozygote, Humans, Infant, Kidney, Male, Mice, Molecular Sequence Data, Mutation, Pedigree, Receptor-Interacting Protein Serine-Threonine Kinases, RNA, Small Interfering, Urinary Tract, Urogenital Abnormalities, Young Adult|
BACKGROUND: Congenital abnormalities of the kidney and the urinary tract are the most common cause of pediatric kidney failure. These disorders are highly heterogeneous, and the etiologic factors are poorly understood.
METHODS: We performed genomewide linkage analysis and whole-exome sequencing in a family with an autosomal dominant form of congenital abnormalities of the kidney or urinary tract (seven affected family members). We also performed a sequence analysis in 311 unrelated patients, as well as histologic and functional studies.
RESULTS: Linkage analysis identified five regions of the genome that were shared among all affected family members. Exome sequencing identified a single, rare, deleterious variant within these linkage intervals, a heterozygous splice-site mutation in the dual serine-threonine and tyrosine protein kinase gene (DSTYK). This variant, which resulted in aberrant splicing of messenger RNA, was present in all affected family members. Additional, independent DSTYK mutations, including nonsense and splice-site mutations, were detected in 7 of 311 unrelated patients. DSTYK is highly expressed in the maturing epithelia of all major organs, localizing to cell membranes. Knockdown in zebrafish resulted in developmental defects in multiple organs, which suggested loss of fibroblast growth factor (FGF) signaling. Consistent with this finding is the observation that DSTYK colocalizes with FGF receptors in the ureteric bud and metanephric mesenchyme. DSTYK knockdown in human embryonic kidney cells inhibited FGF-stimulated phosphorylation of extracellular-signal-regulated kinase (ERK), the principal signal downstream of receptor tyrosine kinases.
CONCLUSIONS: We detected independent DSTYK mutations in 2.3% of patients with congenital abnormalities of the kidney or urinary tract, a finding that suggests that DSTYK is a major determinant of human urinary tract development, downstream of FGF signaling. (Funded by the National Institutes of Health and others.).
|Alternate Journal||N. Engl. J. Med.|
|PubMed Central ID||PMC3846391|
|Grant List||K23-DK090207 / DK / NIDDK NIH HHS / United States |
UM1 HG006504 / HG / NHGRI NIH HHS / United States
P30 DK079310 / DK / NIDDK NIH HHS / United States
1R01DK080099 / DK / NIDDK NIH HHS / United States
K23 DK090207 / DK / NIDDK NIH HHS / United States
DK071041 / DK / NIDDK NIH HHS / United States
U54 HG006504 / HG / NHGRI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
R01 DK053093 / DK / NIDDK NIH HHS / United States
R01 DK080099 / DK / NIDDK NIH HHS / United States
GGP08050 / / Telethon / Italy
HG006504 / HG / NHGRI NIH HHS / United States
R01 DK071041 / DK / NIDDK NIH HHS / United States